Selected Recent Publications: (link to complete list of Engelhardt peer-reviewed publications)
Ritchie T, Zhou W, McKinstry E, Hosch M, Zhang Y, Nathke I, and Engelhardt JF. Developmental expression of catenins and associated proteins during submucosal gland morphogenesis in the airway. Experimental Lung Research 27:121-141, 2001.
Wang X, Zhang Y, Amberson A, and Engelhardt
JF. New models of the tracheal airway define the glandular contribution
to airway surface fluid and electrolyte composition. Am. J. Resp. Cell.
Mol. Biol. 24:195-202, 2001.
Zanetti M, Zwacka RM, Engelhardt JF, Katusic
Z, and O’Brien T. Superoxide anions and endothelial cell proliferation
in normoglycemia and hyperglycemia. Anterioscler Thromb Vasc Biol
21:195-200, 2001.
Sanlioglu S, Monick MM, Luleci G, Hunninghake
GW and Engelhardt, JF. Rate limiting steps of AAV transduction and implications
for human gene therapy. Current Gene Therapy 1:137-147, 2001.
Engelhardt JF. Stem cells niches in the
mouse airway. Am. J. Respir. Cell. Mol. Biol 24:649-652, 2001.
Duan D, Yan Z, Yue Y, Ding W, and Engelhardt
JF. Enhancement of Muscle Gene Delivery with Pseudotyped Adeno-Associated
Virus Type 5 Correlates with Myoblast Differentiation. Journal of Virology
75(16):7662-7671, 2001.
Li Q, Sanlioglu S, Li S, Ritchie T, Oberley
L, and Engelhardt JF. GPx-1 gene delivery modulates NFkB activation following
diverse environmental injuries through a specific subunit of the IKK complex.
Antioxidant and Redox Signaling 3(3):415-432, 2001.
Engelhardt JF, Sen CK, and Oberley L. Redox-modulating
gene therapies for human diseases Antioxidant and Redox Signaling
3(3):341-346, 2001.
Zhou W, Zhang Y, Hosch MS, Lang A, Zwacka
RM, and Engelhardt JF. Subcellular site of superoxide dismutase expression
differentially controls AP-1 activity in the liver and injury following
ischemia/reperfusion. Hepatology 33(4):902-914, 2001.
Sanlioglu S, Williams C, Samavati L, Butler
N, Wang G, McCray P Jr., Ritchie T. Hunninghake G, Zandi E, and Engelhardt
JF. Lipopolysaccharide Induces Rac1-dependent Reactive Oxygen Species
Formation and Coordinates Tumor Necrosis Factor- a Secretion through IKK
Regulation of NF- kB. J Biolog Chem 276(32): 30188-30198, 2001.
Duan D, Yue Y, and Engelhardt JF. Expanding
AAV Packaging Capacity with Trans-splicing or Overlapping Vectors: A Quantitative
Comparison. Molecular Therapy 4(4):383-391, 2001.
Walters RW, Duan D, Engelhardt JF, and Welsh
MJ. Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate
Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo. J.
Virology, 74:535-540, 2000.
Naren AP, Anke D, Cormet-Boyaka E, Boyaka
PN, McGhee JR, Zhou W, Akagawa K, Jujiwara T, Thome U, Engelhardt JF,
Nelson DJ, and Kirk KL. Syntaxin 1A is Expressed in Airway Epithelial
Cells Where it Modulates CFTR Cl- Currents. J. Clin. Invest. 105:377-386,
2000.
Sanlioglu S, Benson P, and Engelhardt JF.
Loss of ATM Function Enhances Recombinant Adeno-Associated Virus Transduction
and Integration through Pathways Similar to UV Irradiation. Virology,
268:68-78, 2000. (Cover Image)
Duan D, Yue Y, Yan Z, and Engelhardt JF.
A New Dual Vector Approach to Enhance Recombinant AAV Mediated Gene Expression
Through Intermolecular Cis-activation. Nature Med. 6:595-598, 2000.
Yan Z, Zhang Y, Duan D, and Engelhardt JF.
Trans-Splicing Vectors Expand the Utility of Adeno-Associated Virus for
Gene Therapy. PNAS 97:6716-6721, 2000.
Jiang Q, Li J, Dubroff R, Ahn YJ, Foskett,
JK, Engelhardt JF, Kleyman, TR. Epithelial sodium channels regulate cystic
fibrosis transmembrane conductance regulator chloride channels in Xenopus
oocytes. J. Biol. Chem. 275:13266-13274, 2000.
Duan D, Yue Y, Yan Z, Yang J, and Engelhardt
JF. Endosomal Processing Limits Gene Transfer to Polarized Airway Epithelia
by Adeno-Associated Virus. J. Clin. Invest. 105:1573-1587, 2000.
(Cover Image)
Lam EWN, Hammad HM, Zwacka R, Darby CJ,
Baumgardner KR, Davidson BL, Oberley TD, Engelhardt JF, and Oberley LW.
Immunolocalization and adenoviral vector-mediated manganese superoxide
dismutase gene transfer to experimental oral tumors. J. Dent. Res.
79:1410-1417, 2000.
Sanlioglu, S., P.K. Benson, J. Yang, E.M.
Atkinson, T. Reynolds, and J.F. Engelhardt. Endocytosis and nuclear trafficking
of adeno-associated virus type 2 are controlled by rac1 and phosphatidylinositol-3
kinase activation. J Virol. 74:9184-9196. 2000.
Epperly, MW, Bray, JA, Krager, S, Berry,
LM, Gooding, W, Engelhardt, JF, Zwacka, R, Travis,EL, and Greenberger,
JS Intratracheal injection of adenovirus containing the human MnSOD transgene
protects athymic nude mice from irradiation-induced organizing alveolitis.
Int. J. Radiation Oncology Biol. Phys. 43:169-181, 1999.
Duan D, Sharma P, Dudus L, Zhang Y, Sanlioglu
S, Yan Z, Yue Y, Ye Y, Lester R, Yang J, Fisher KJ and Engelhardt JF.
Formation of Adeno-Associated Virus Circular Genomes is Differentially
Regulated by Adenovirus E4-ORF6 and E2a Gene Expression. J. Virology
73:161-169, 1999.
Zhang Y and Engelhardt JF. Airway Surface
Fluid Volume and Chloride Content in Cystic Fibrosis and Normal Bronchial
Xenografts. Am. J. Physiol: Cell Physiol 276:C469-C476, 1999.
Sanlioglu S, Duan D, and Engelhardt JF.
Two independent molecular pathways for rAAV genome conversion occur following
UV-C and E4orf6 augmentation of rAAV transduction. Human Gene Therapy
10:591-602, 1999.
Duan D, Yue Y, and Engelhardt JF: Response
to “Polarity Influences the efficiency of recombinant adeno-associated
virus infection in differentiated airway epithelia”. Human Gene
Therapy 10:1553-1557, 1999.
Sanlioglu, S, and Engelhardt JF. Cellular
Redox State Alters Recombinant Adeno-Associated Virus Transduction Through
Tyrosine Phosphatase Pathways. Gene Therapy 6:1427-1437, 1999.
Duan, D, Yan, Z, Yue, Y, and Engelhardt,
JF. Structural analysis of AAV transduction circular intermediates. Virology
261:8-14, 1999.
Lam EW, Zwacka R, Seftor EA, Nieva DRC,
Davidson BL, Engelhardt JF, Hendrix MJC, and Oberley LW. Effects of antioxidant
enzyme overexpression on the invasive phenotype of hamster cheek pouch
carcinoma cells. Free Radical Biol. & Med. 27:572-579, 1999.
Yang J, Zhou W, Zhang Y, Zidon T, Ritchie
T, and Engelhardt JF: Concatamerization of Adeno-Associated Virus Circular
Genomes Occurs through Intermolecular Recombination. J Virology.
73:9468-9477, 1999.
Duan D, Yue Y, Zhou W, Labed B, Ritchie
T, Grosschedl R, and Engelhardt JF: Submucosal Gland Development in the
Airway is Controlled by Lymphoid Enhancer Binding Factor-1 (Lef-1). Development,
126:4441-4453, 1999.
Duan D, Li Q, Kao A, Yue Y, Pessin J, and
Engelhardt JF: Dynamin is Required for Recombinant Adeno-associated Virus
Type 2 Infection. J Virology, 73:10371-10376, 1999.
Sharma P, Dudus L, Nielsen PA, Clausen H,
Yankaskas JR, Hollingworth MA, and Engelhardt JF: MUC5B and MUC7 are Differentially
Expressed in Mucous and Serous Cells of Submucosal Glands in Human Bronchial
Airways. Am. J. Resp. Cell. Mol. Biol. 19:30-37, 1998. (Cover Image)
Duan D, Sehgal A, Yao J, and Engelhardt
JF: The Lef1 transcription factor expression defines airway progenitor
cell targets for in utero gene therapy of submucosal gland in cystic fibrosis.
Am. J. Resp. Cell. Mol. Biol. 18:750-758, 1998.
Zwacka RM, Dudus L, Epperly MW, Greenberger
JS, and Engelhardt JF: Redox Gene Therapy Protects Human IB-3 Lung Epithelial
Cells Against Ionizing Radiation-Induced Apoptosis. Human Gene Therapy
9:1381-1386, 1998.
Zhang Y, Jiang Q, Dudus, L, Yankaskas JR,
and Engelhardt JF: Vector Specific Complementation Profiles of Two Independent
Primary Defects in Cystic Fibrosis Airways. Human Gene Therapy
9:635-648, 1998.
Gonzalez-Zulueta, M, Mukhin G, Zwacka RM,
Engelhardt JF, Oberley LW, Dawson VL, and Dawson TM: Manganese Superoxide
dismutase protects nNOS Neurons from NMDA and Nitric Oxide Mediated Neurotoxicity.
J. Neuroscience 18:2040-2055, 1998.
Epperly M, Bray J, Kraeger S, Swacka R,
Engelhardt JF, Travis E, and Greenberger J: Prevention of Late Effects
of Irradiation Lung Damage by Manganese Superoxide Dismutase Gene Therapy.
Gene Therapy 5:196-208, 1998.
Fang X, Weintraub NL, Rios CD, Chappell
DA, Zwacka RM, Engelhardt JF, Oberley LW, Yan T, Heistad DD, and Spector
AA: Overexpression of Human Superoxide Dismutase Inhibits Oxidation of
Low-Density Lipoprotein by Endothelial Cells. Circulation Research
82:1289-1297, 1998.
Zwacka RM, Zhou W, Zhang Z, Darby CJ, Dudus
L, Halldorson J, Oberley L, and Engelhardt JF: Redox Gene Therapy of Liver
Ischemia/Reperfusion Injury Reduces AP1 and NFkB Activation. Nature
Medicine 4:698-704, 1998.
Duan D, Sharma P, Yang J, Y Yue, Dudus L,
Zhang Y, Fisher KJ, and Engelhardt JF: Circular Intermediates of Recombinant
Adeno-Associated Virus have Defined Structural Characteristics Responsible
for Long Term Episomal Persistence In Muscle Tissue. J. Virology
72:8568-8577, 1998.
Zwacka RM, Zhang Y, Halldorson J, and Engelhardt
JF: Ischemia/reperfusion injury in the liver of BALB/c mice activates
AP-1 and NF-kB Independently of IkB Degradation. Hepatology 28:1022-1030,
1998.
Jiang Q, Mak D, Devidas S, Schwiebert EM,
Bragin A, Zhang Y, Skach WR, Guggino WB, Foskett JK, and Engelhardt JF:
Cystic fibrosis transmembrane conductance regulator-associated ATP release
is controlled by a chloride sensor. J. of Cell Biology 143(3):645-57,
1998.
Ceneviva GD, Tzeng E, Hoyt DG, Yee E, Gallagher
A, Engelhardt JF, Kim YM, Billiar TR, Watkins SA, and Pitt BR. Nitric
oxide inhibits lipopolysaccharide-induced apoptosis in pulmonary artery
endothelial cells. Am. J. Physiol 275:L717-28, 1998.
Duan D, Yue Y, McCray PB, and Engelhardt
JF: Polarity Influences the efficiency of
recombinant adeno-associated virus infection in differentiated airway
epithelia. Human Gene Therapy 9:2761-2776, 1998.
Woo YJ, Zhang JC, Vijayasarathy C, Zwacka
RM, Engelhardt JF, Gardner TJ, Sweeney HL. Recombinant adenovirus-mediated
cardiac gene transfer of superoxide dismutase and catalas attenuates postischemic
contractile dysfunction. Circulation 19:II255-60, 1998.
Recent Review Articles:
Engelhardt JF, Sen CK, Oberley L. "Redox-modulating
gene therapies for human diseases." Antioxid Redox Signal Jun;3(3):341-6,
2001.
Engelhardt, JF. "Redox Mediated Gene Therapies
for Environmental Injury: Approaches and Concepts", in Antioxidant
and Redox Signaling 1:5-27, 1999.
Fan C, Zwacka RM, and Engelhardt JF. "Therapeutic Approaches for
Ischemia/Reperfusion Injury in the Liver", in Journal of Molecular
Medicine 77:577-92, 1999.
Jiang Q and Engelhardt JF. "Cellular Heterogeneity
of CFTR Expression and Function in the Lung: Implications for Gene Therapy",
European Journal of Human Genetics 6:12-31, 1998.
Duan D, Zhang Y, and Engelhardt JF. "Gene Delivery to the Airway",
in Current Protocols in Human Genetics, John Wiley & Sons, Inc., 1998.
Engelhardt JF. "Methods of Adenoviral-Mediated
Gene Transfer to Airway Epithelium", in Methods in Molecular Biology:
Gene Therapy Protocols, Humana Press, Inc., 1996.
Engelhardt JF and Wilson JM. "Explant Models of the Airway",
in The Lung, Raven Press, Inc., eds. Crystal and West, 1996.
